Colchicine (a high-priced alkaloid) accumulation and HPTLC quantification in different stages of in vitro developed tuber of Gloriosa superba L.

Future Journal of Pharmaceutical Sciences

Table 1 In vitro tuber formation in Gloriosa

Hormones types	Concentration (mg/l)	% Growth response	No. of microtubers/explants (Mean ± SE)
Control	–	2	2.26 ± 1.1^f
BA	1	–	–
	2	–	–
	3	90	37.7 ± 0.5^a
BA + NAA	1 + 0.2	50	26.3 ± 1.5^c
	2 + 0.2	60	31.3 ± 1.1^b
	3 + 0.2	20	25.0 ± 1.7^c
Kin. + 2,4-D	0.2 + 0.5	40	21.6 ± 0.5^d
	0.5 + 1.0	70	30.3 ± 1.5^b
	1 + 1.5	30	14.3 ± 1.1^e

Data represent the mean ± standard error of 10 replicates for each treatment. Means were compared using DMRT (Duncan’s Multiple Range Test) (P ≤ 0.05) using one-way ANOVA. Mean values within the column with the same letter in superscript are not significantly different [34]