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Table 2 Optimized chromatographic conditions of empagliflozin

From: Stability-indicating HPLC-DAD method for the determination of empagliflozin

Parameters Conditions
Stationary phase C18 (4.6 × 100 mm, 4 µm)
Mobile phase Methanol/Acetonitrile/0.1%OPA (75:20:5)
Flow rate (mL/min) 1.0
Run time (min) 10
Detection wavelength (nm) 222.0
Injection volume (µL) 20
Retention time (min) 2.54