Table 2 Optimized chromatographic conditions of empagliflozin
From: Stability-indicating HPLC-DAD method for the determination of empagliflozin
Parameters | Conditions |
|---|---|
Stationary phase | C18 (4.6 × 100 mm, 4 µm) |
Mobile phase | Methanol/Acetonitrile/0.1%OPA (75:20:5) |
Flow rate (mL/min) | 1.0 |
Run time (min) | 10 |
Detection wavelength (nm) | 222.0 |
Injection volume (µL) | 20 |
Retention time (min) | 2.54 |