Table 4 Robustness studies of proposed method
Normal Condition | Changed condition | MET (200 µg/ml) (n = 3) | LINA (1 µg/ml) (n = 3) | EMPA (2 µg/ml) (n = 3) | |||
|---|---|---|---|---|---|---|---|
Rt mean ± SD | %RSD | Rt mean ± SD | %RSD | Rt mean ± SD | %RSD | ||
Wavelength 223 nm | 221 | 2.14 ± 0.03 | 1.63 | 3.37 ± 0.06 | 1.80 | 14.3 ± 0.15 | 1.06 |
225 | 2.08 ± 0.03 | 1.66 | 3.36 ± 0.05 | 1.71 | 14.3 ± 0.15 | 1.06 | |
Flow rate 1 ml/min | 0.9 ml/min | 2.15 ± 0.03 | 1.67 | 3.43 ± 0.05 | 1.51 | 14.23 ± 0.12 | 0.8 |
1.1 ml/mi | 1.80 ± 0.01 | 0.84 | 2.87 ± 0.04 | 1.60 | 13.04 ± 0.25 | 1.95 | |
Acetonitrile and Methanol (HPLC Grade) SRL Pvt.Ltd | Acetonitrile and Methanol (HPLC Grade) E-Merck Pvt. Ltd | 2.06 ± 0.03 | 1.55 | 3.44 ± 0.06 | 1.74 | 14.35 ± 0.21 | 1.47 |
Acetonitrile and Methanol (HPLC Grade) Rankem Pvt. Ltd | 2.06 ± 0.04 | 1.94 | 3.44 ± 0.05 | 1.70 | 14.36 ± 0.15 | 1.06 | |