Table 1 Summary of method conditions

Eluent

0.1% Formic acid in water/acetonitrile—50/50 (v/v)

Flow rate

1.0 mL min⁻¹

Auto-sampler temperature

25 °C

Injection volume

20 µL

Column temperature

30 °C

Elution

Isocratic

Run time

10 min

Source

ESI

Ionization mode

Positive

Capillary voltage

5 kV

Cone voltage

30 V

Acquisition mode

MRM

Parent m/z selection for quantification

125.1 Da

Source temperature

150 °C

Desolvation temperature

320 °C

Drying and nebulization gas

Nitrogen

Nebulization gas flow

1.5 mL min⁻¹

Drying gas flow

15 mL min⁻¹

CID gas

Argon

Collision energy

40 V