Table 3 Summary of HPLC method validation parameters
From: Characterization and In-silico toxicity prediction of degradation products of felbamate
Validation parameters | Results |
|---|---|
Linearity | |
Equation of the regression line | *y = 12947x − 26459 |
r2 | 0.9991 |
Range (µg mL−1) | 20–120 |
Precision | |
Intraday | |
µg mL−1 | Mean peak area ± SD; %CV |
40 | 489,900 ± 1353, 0.3 |
50 | 631,412 ± 4068, 0.4 |
60 | 750,941 ± 10,430, 1.4 |
Inter-day | |
40 | 491,919 ± 10,430, 0.3 |
50 | 633,906 ± 9433, 1.5 |
60 | 743,905 ± 4482, 0.6 |
Accuracy | |
Spiked concentration | |
µg mL−1 | % recovery mean ± SD, %CV |
40 | 40.4 ± 0.4, 1.0 |
50 | 49.4 ± 0.6,1.3 |
60 | 60.4 ± 0.5,0.9 |