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Table 1 Different trails

From: A new stability indicating RP-UPLC method for simultaneous estimation of Doravirine, Lamivudine and Tenofovir disoproxil fumarate in bulk and their combined pharmaceutical formulation

Trail

Column

Mobile phase (% v/v)

Flow rate

Observation

1

STD Hibar C18

(100 × 2.1 mm, 2 µm)

Acetonitrile:Water (50:50)

0.3 mL

Baseline noise was observed and platecount of Doravirine peak was less than 2000 (1883)

2

Standard Hibar C18 (100 × 2.1 mm, 2µ)

Methanol:Water (50:50)

0.3 mL

Tenofovir was not eluted and resolution between Doravirine and Lamivudine was less than 2

3

STD Hibar C18 (100 × 2.1 mm, 2 µm)

Acetonitrile:OPA (50:50)

0.3 mL

Doravirine peak plate count was less than 2000 (847.8) and Lamivudine plate count was near to 2000 (2003.6)

4

Standard SB C18 (100 × 2.1 mm, 2µ)

Acetonitrile: Kh2po4 (40:60)

0.3 mL

Doravirine peak plate count was lessthan 2000 (1275.7)

5

HSS C18 (100 × 2.1 mm, 2µ)

Acetonitrile: Kh2po4 (40:60)

0.3 mL

Doravirine, Lamivudine and Tenofovir disoproxil fumarate were eluted at retention time of 1.215, 1.498, 1.823 min respectively

  1. v/v volume by volume, STD standard, SB stable bonding, HSS hollow structural sections, mm millimeter, mL milliliter, % percentage, µm micrometer, OPA ortho phosphoric acid, Kh2po4 potassium dihydrogen orthophosphate buffer