Trail | Column | Mobile phase (% v/v) | Flow rate | Observation |
---|---|---|---|---|
1 | STD Hibar C18 (100 × 2.1 mm, 2 µm) | Acetonitrile:Water (50:50) | 0.3 mL | Baseline noise was observed and platecount of Doravirine peak was less than 2000 (1883) |
2 | Standard Hibar C18 (100 × 2.1 mm, 2µ) | Methanol:Water (50:50) | 0.3 mL | Tenofovir was not eluted and resolution between Doravirine and Lamivudine was less than 2 |
3 | STD Hibar C18 (100 × 2.1 mm, 2 µm) | Acetonitrile:OPA (50:50) | 0.3 mL | Doravirine peak plate count was less than 2000 (847.8) and Lamivudine plate count was near to 2000 (2003.6) |
4 | Standard SB C18 (100 × 2.1 mm, 2µ) | Acetonitrile: Kh2po4 (40:60) | 0.3 mL | Doravirine peak plate count was lessthan 2000 (1275.7) |
5 | HSS C18 (100 × 2.1 mm, 2µ) | Acetonitrile: Kh2po4 (40:60) | 0.3 mL | Doravirine, Lamivudine and Tenofovir disoproxil fumarate were eluted at retention time of 1.215, 1.498, 1.823 min respectively |