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Table 2 Gradient program

From: Impurity profiling method development and validation of metformin hydrochloride and teneligliptin hydrobromide hydrate in their combination tablet dosage form by using RP-HPLC with UV/PDA detector

Time in min

% mobile phase A

% mobile phase B

0.1

75

25

10.0

70

30

45.0

40

60

55.0

40

60

55.01

75

25

65.0

75

25