|
Chromatographic changes
|
*Peak area
|
Tailing Factor
|
Theoretical Plates
|
*Assay (% w/w) ± SD, % RSD
|
|---|
| | |
BA
|
EZ
|
BA
|
EZ
|
BA
|
EZ
|
BA
|
EZ
|
|---|
|
Flow rate ± 0.1 (mL/min.)
|
0.4
|
45,118,072
|
12,608,841
|
1.184
|
1.407
|
3588
|
9450
|
100.59 ± 0.1799, 0.18
|
99.91 ± 0.3205, 0.32
|
| |
0.5
|
44,965,324
|
12,588,406
|
1.172
|
1.166
|
3041
|
7911
| | |
| |
0.6
|
45,051,231
|
12,583,731
|
1.144
|
1.123
|
2455
|
7299
| | |
|
Temperature ± 5 (°C)
|
20
|
45,036,493
|
12,584,203
|
1.155
|
1.163
|
3481
|
9827
|
100.51 ± 0.2184, 0.22
|
99.88 ± 0.4116, 0.41
|
| |
25
|
44,906,494
|
12,580,784
|
1.181
|
1.173
|
3112
|
8067
| | |
| |
30
|
45,086,495
|
12,612,046
|
1.108
|
1.122
|
2484
|
6857
| | |
|
Detection
wavelength ± 2 (nm)
|
258
|
44,934,914
|
12,597,848
|
1.153
|
1.141
|
3585
|
9708
|
100.53 ± 0.1820, 0.18
|
99.60 ± 0.3573, 0.34
|
| |
260
|
45,040,389
|
12,569,963
|
1.158
|
1.174
|
3088
|
8102
| | |
| |
262
|
45,085,863
|
12,574,861
|
1.134
|
1.128
|
2548
|
6989
| | |
- *Mean of three determinations; SD = Standard Deviation; RSD = Relative Standard Deviation
