Fig. 3
From: Evaluation of anti-epithelial-mesenchymal transition property of Garcinia mangostana rind extract
Mangosteen rind extract (MRE)-induced expression of hormone receptors and EMT markers in MCF-7 cells. A Messenger RNA expression of ERα, ERβ, EGFR, and EMT markers in MCF-7 cells. MCF-7 cells were treated with MRE (0, 50, and 100 μg/ml) and the effect of MRE on gene expression were studied. Messenger-RNA expressions were analyzed using real-time PCR and the experiments were done in triplicates. ERα = Estrogen receptor alpha, ERβ = Estrogen receptor beta, EGFR = Epidermal growth factor receptor, SNAIL = Zinc finger protein SNAI1 (Snail), EMT = Epithelial-mesenchymal Transition, MMP = Matrix metalloproteases. B Effect of MRE on activity of MMP-9 in MCF-7 cells. MMP-9 activity was analyzed using Gelatin zymography. The dark bands against the light background (reversed from original image) showed the activities of pro and active MMP-9