Table 1 List of primers used for quantitative real-time PCR of genes
From: Evaluation of anti-epithelial-mesenchymal transition property of Garcinia mangostana rind extract
S. no | Gene | 5′ → 3′ sequence | |
|---|---|---|---|
1 | ER alpha | F | TGGGCTTACTGACCAACCTG |
R | CCTGATCATGGAGGGTCAAA | ||
2 | ER beta | F | AGAGTCCCTGGTGTGAAGCAA |
R | GACAGCGCAGAAGTGAGCATC | ||
3 | EGFR | F | CTAAGATCCCGTCCATCGCC |
R | GGAGCCCAGCACTTTGATCT | ||
4 | E Cadherin | F | GCCGCTGGCGTCTGTAGGAA |
R | TGACCACCGCTCTCCTCCGA | ||
5 | N Cadherin | F | TCGCCATCCAGACCGACCCA |
R | TGAGGCGGGTGCTGAATTCCC | ||
6 | SNAIL | F | ATGCCGCGCTCTTTCCTCGTC |
R | AGCAGGTGGGCCTGGTCGTAG | ||
7 | GAPDH | F | TCAAGTGGGGCGATGCTGGC |
R | TGACCTTGGCCAGGGGTGCT | ||