Parameters | Results |
---|---|
Type of system | HPLC with UV detector or equivalent |
Pump mode | Isocratic |
Mobile phase | 10 mM phosphate buffer with 1.1 g octane-1-sulfonic acid sodium salt having pH 2.5 (adjusted with 5% OPA) and Acetonitrile (500:500, v/v) |
Column | Oyster ODS3 (5 µm, 4.6 × 150 mm) P/N: S670153 (Make: Merck & Co.) |
Injection volume | 20 µL |
Run time | 9 min |
Flow rate | 1.0 mL/min |
Detection | 242.0 nm |
Column temperature | Ambient (about 25 °C) |
Blank | Diluent |