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Table 7 Method validation results of Bempedoic acid and Ezetimibe by UPLC

From: Characterization of novel stress degradation products of Bempedoic acid and Ezetimibe using UPLC–MS/MS: development and validation of stability-indicating UPLC method

Parameter Bempedoic acid Ezetimibe
Concentration (µg/ml) Result Concentration (µg/ml) Result
Linearity 18–360 CC: 0.999 1–20 CC: 0.999
Accuracy 90 % Rec: 100.1 5 %Rec: 99.5
180 %Rec: 99.9 10 % Rec: 99.3
270 % Rec: 99.6 15 % Rec: 99.9
Intraday precision 180 %RSD: 0.87 10 %RSD: 0.65
Interday precision 180 %RSD: 0.36 10 %RSD: 0.61
Robustness     
Flow Plus
Flow Minus
180
180
0.41
0.37
10
10
0.33
0.82
Organic Plus 180 0.78 10 0.51
Organic Minus 180 0.52 10 0.78
Wavelength Plus 180 0.42 10 0.84
Wavelength Minus 180 0.39 10 0.92
  1. CC correlation coefficient
  2. % REC-% Recovery
  3. %RSD: Relative standard deviation