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Table 2 Results of the validation parameters

From: A novel simultaneous high performance liquid chromatography-PDA method for the determination of Tenofovir AF, Darunavir, Emtricitabine and Cobicistat in bulk and its application to marketed formulation

Parameters

Tenofovir

Darunavir

Emtricitabine

Cobicistat

Precision (%RSD)

1.0

0.6

0.8

0.6

Intermediate precision (%RSD)

0.2

0.8

0.2

0.9

Accuracy (Mean recovery)

100.11

100.26

100.64

100.08

Linearity (µg/mL)

2–10

160–800

40–200

30–150

Regression coefficient

0.999

0.999

0.999

0.999

LOD (µg/mL) (signal to noise ratio method)

0.14

2.14

0.6

7.32

LOQ(µg/mL) (signal to noise ratio method)

0.47

7.12

2.10

24.42