Table 3 Results of system suitability

Theoretical plates (N)

6251

6400

Mean (n = 6) > 2000

Capacity factor (K´)

1.85

3.23

K′ > 2.0

Resolution (Rs)

–

8.25

 > 2 between NAP and ESP

Tailing factor (TF)

1.06

1.00

Mean (n = 6) ≤ 2

Repeatability

0.05

0.21

% RSD (n = 6) < 2.00

Retention time (RT)

0.27

0.20

% RSD (n = 6) < 2.00