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Table 1 Selected factor combinations for Piperine as per 22 full factorial design

From: Quality assessment and Analytical Quality by Design-based RP-HPLC method development for quantification of Piperine in Piper nigrum L.

Code Coded levels Actual values Responses
X1 X2 X1 (%) X2 R1 R2
T1  − 1 − 1 0.05 60:40 1.31 0.49
T2 − 1 + 1 0.05 70:30 1.23 0.43
T3 + 1 + 1 1 70:30 1.47 0.55
T4 + 1 − 1 1 60:40 1.57 0.62
  1. X1-conc. of acetic acid (%); X2- mobile phase ratio, R1-tailing factor, R2- peak width