Future Journal of Pharmaceutical Sciences

Table 1 Selected factor combinations for Piperine as per 2² full factorial design

From: Quality assessment and Analytical Quality by Design-based RP-HPLC method development for quantification of Piperine in Piper nigrum L.

Code	Coded levels		Actual values		Responses
Code	X1	X2	X1 (%)	X2	R1	R2
T1	− 1	− 1	0.05	60:40	1.31	0.49
T2	− 1	+ 1	0.05	70:30	1.23	0.43
T3	+ 1	+ 1	1	70:30	1.47	0.55
T4	+ 1	− 1	1	60:40	1.57	0.62

X1-conc. of acetic acid (%); X2- mobile phase ratio, R1-tailing factor, R2- peak width

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