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Future Journal of Pharmaceutical Sciences

Table 2 Summary of statistical parameters and polynomial equation

From: Quality assessment and Analytical Quality by Design-based RP-HPLC method development for quantification of Piperine in Piper nigrum L.

Response

P value

Model significance

Polynomial equation

Piperine

R1

0.0376

Significant

+ 1.39 + 0.1250 * X1 − 0.0450 * X2

R2

0.0355

Significant

+ 0.5225 + 0.0625 * X1 − 0.0325 * X2

  1. X1 and X2 are independent variables where, X1—conc. of acetic acid in aqueous phase and X2—mobile phase ratio
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