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Table 3 Optimized chromatographic conditions

From: Quality assessment and Analytical Quality by Design-based RP-HPLC method development for quantification of Piperine in Piper nigrum L.

Parameters Chromatographic conditions
Stationary Phase ZORBAX C-18 (250 mm × 4.6 mm, 5 µ) column
Mobile phase Acetonitrile:Water (0.05% acetic acid)
Mobile phase ratio 70:30
Flow rate 1.00 mL/min
Detection wavelength 342.00 nm
Injection volume 20.00 µL
Retention time 5.5 min