Table 3 Optimized chromatographic conditions
Parameters | Chromatographic conditions |
|---|---|
Stationary Phase | ZORBAX C-18 (250 mm × 4.6 mm, 5 µ) column |
Mobile phase | Acetonitrile:Water (0.05% acetic acid) |
Mobile phase ratio | 70:30 |
Flow rate | 1.00 mL/min |
Detection wavelength | 342.00 nm |
Injection volume | 20.00 µL |
Retention time | 5.5 min |