Table 4 Summary of validation parameters
Validation parameters | Piperine |
|---|---|
System suitability | |
Retention time | |
Mean ± SD | 5.54 ± 0.003 |
% RSD | 0.05 |
Peak area | |
Mean ± SD | 20,156,202 ± 120,229 |
% RSD | 0.59 |
Tailing factor | |
Mean ± SD | 1.24 ± 0.01 |
% RSD | 0.68 |
Linearity | |
Linearity range (µg/mL) | 5.00–25.00 |
Correlation-coefficient | 0.9986 |
LOD (µg/mL) | 1.12 |
LOQ (µg/mL) | 3.41 |
Precision | |
Intra-day (%RSD) | 1.35 |
Inter-day (%RSD) | 1.56 |
Accuracy | |
80% | |
% Recovery | 99.85 ± 0.28 |
100% | |
% Recovery | 101.20 ± 0.02 |
120% | |
% Recovery | 96.35 ± 0.05 |