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Table 4 Summary of validation parameters

From: Quality assessment and Analytical Quality by Design-based RP-HPLC method development for quantification of Piperine in Piper nigrum L.

Validation parameters Piperine
System suitability
Retention time
 Mean ± SD 5.54 ± 0.003
 % RSD 0.05
Peak area
 Mean ± SD 20,156,202 ± 120,229
 % RSD 0.59
Tailing factor
 Mean ± SD 1.24 ± 0.01
 % RSD 0.68
Linearity
Linearity range (µg/mL) 5.00–25.00
Correlation-coefficient 0.9986
LOD (µg/mL) 1.12
LOQ (µg/mL) 3.41
Precision
Intra-day (%RSD) 1.35
Inter-day (%RSD) 1.56
Accuracy
80%
 % Recovery 99.85 ± 0.28
100%
 % Recovery 101.20 ± 0.02
120%
 % Recovery 96.35 ± 0.05