Potentials of N-(2, 4-dinitro-1-naphthyl)-p-toluenesulphonamide and 2, 4-dinitro-1-naphthol as novel charge transfer acceptors in pharmaceutical analysis of some quinolones and cephalosporins

Future Journal of Pharmaceutical Sciences

Table 2 Spot test results and chromatographic R_f values for N-(2, 4-DN1NL) PTS-azo adduct formed with drugs studied

Drugs	Colour of adducts at 30 °C		Colour of adducts at 60 °C		R_f values ** (CHCl₃: EtOAc: MeOH (4:4:2 v/v)
Drugs	Instantly	5 min	5 min	20 min	R_f values ** (CHCl₃: EtOAc: MeOH (4:4:2 v/v)
Sparfloxacin	Deep golden yellow	Deep golden yellow	Deep golden yellow	Deep golden yellow	0.35 and 0.83 (0.35)
Norfloxacin	Deep golden yellow	Deep golden yellow	Deep golden yellow	Deep golden yellow	0.043 and 0.83 (0.043)
Ofloxacin	Deep golden yellow	Deep golden yellow	Deep golden yellow	Deep golden yellow	0.043 and 0.83 (0.043)
Ceftriaxone	Deep golden yellow	Deep golden yellow	Deep golden yellow	Deep golden yellow	0.014 and 0.83 (0.014)
Ceftazidime	Deep golden yellow	Deep golden yellow	Deep golden yellow	Deep golden yellow	0.014 and 0.83 (0.014)
Cefuroxime	Deep golden yellow	Deep golden yellow	Deep golden yellow	Deep golden yellow	0.014 and 0.83 (0.014)

*Blank reagent solution without drug is lemon yellow; **R_f of drug substance in parenthesis (R_f of reagent = 0.83)