Table 1 Summary of validation data

Linear range [µg/mL] [n = 5]

4–24 µg/mL

Correlation coefficient

0.9998

Limit of detection [µg/mL]

0.35

Limit of quantification [µg/mL]

1.08

% Recovery [n = 3]

99.41–99.80

Precision [%RSD]

Repeatability [n = 6]

0.27

Intra-day [n = 3]

0.28–0.61

Inter-day [n = 3]

0.04–0.25

Ruggedness [% RSD]

Analyst – I [n = 6]

0.54

Analyst – II [n = 6]

0.51

Robustness

Robust