From: Experimental design approach, screening and optimization of system variables, analytical method development of flurbiprofen in nanoparticles and stability-indicating methods for high-pressure liquid chromatography
Independent variables
Level used,
actual (coded)
(− 1)
(0)
(+ 1)
X1 = Flow rate (ml/min)
1
1.1
1.2
X2 = Temperature (°C)
20
22.5
25
X3 = Methanol (%)
80
82.5
85