Table 1 Different trials
Trail | Column | Buffer | Mobile phase | Flow rate ml/min | Observation |
|---|---|---|---|---|---|
1 | ODS (150 × 4.6 mm, 5 μm) | 0.1% TFA in water | Buffer: methanol (40:60) | 1 | Peaks were not good |
2 | Xterra RPC18 (150 × 4.6 mm, 5 μm) | 0.1% formic acid in water | Buffer: ACN (50:50) | 1 | Broad peak for Dolutegravir |
3 | Xterra RPC18 (150 × 4.6 mm, 5 μm) | 0.1% formic acid in water | Buffer: ACN (20:80) | 1 | Peak of Dolutegravir was not good |
4 | Phenyl XDB (250 × 4.6 mm, 5 μm) | 0.1% TFA in water | Buffer: ACN (40:60) | 1 | Resolution of peaks was not good, and peak of Dolutegravir was not good |
5 | Phenyl XDB (250 × 4.6 mm, 5 μm) | 0.1% TFA in water | Buffer: methanol (50:50) | 0.8 | No peak is observed for Dolutegravir |
6 | Phenyl XDB (250 × 4.6 mm, 5 μm) | 0.1% TFA in water | Buffer: methanol (20:80) | 0.8 | Long RT of Dolutegravir |
7 | Phenyl XDB (250 × 4.6 mm, 5 μm) | 0.1% TFA in water | Buffer: methanol (15:85) | 0.8 | Good resolution and good shape of peaks |