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Table 7 Comparison of the developed HPLC method and the reported methods for analysis of MLC in plasma samples

From: Meloxicam quantification in rabbit plasma by RP-HPLC: optimization and application to pharmacokinetic study

Author name(s) Method Linearity LOD Extraction method adopted % Recovery Mobile phase Plasma Reference number
Miyamoto A HPLC-UV 20 to 1000 ng mL−1 Not performed Solid phase extraction 92.80 % 50 mM phosphate buffer (pH 2.15, solvent A) and acetonitrile (solvent B) at a flow rate of 0.8 mL/min. Isocratic elution (solvent A:solvent B = 60:40) Rat 1
Dasandi B, Shivaprakash, Saroj H, Bhat KM HPLC 50 to 1500 ng mL−1 10 ng mL−1 Protein precipitation More than 85% Sodium acetate buffer (pH 3.3, 170 mmol):acetonitrile (62:38 v/v) mixture Human 4
Bae JW, Kim MJ, Jang CG, Lee SY HPLC 10–2400 ng/mL Liquid-liquid extract Maximum 86.7 % Acetonitrile–20mM potassium hydrogen phosphate (40:60, v/v, pH 3.5) Human 5
Hye YJ, Hye WL, Young HK, et al LC–MS/MS 0.50–200 (ng/mL) Liquid-liquid extraction 87.1 % maximum Methanol:ammonium formate (15 mM, pH 3.0) (60:40, v/v) Human 7
Pairis-Garcia MD, Johnson AK, Kukanich B, et al HPLC-MS 0.005 μg/mL Protein precipitation A. 0.1% formic acid in water and B. 0.1% formic acid in an acetonitrile at a flow rate of 0.250 mL/min Swine 10
Shukla M, Singh G, Sindhura BG, et al HPLC 0.02
μg mL−1
Protein precipitation 89.00–93.00 %. 65% water:acetic acid (99:1, v/v) and 35% acetonitrile Human 12
Velpandian T, Jaiswal J, Bhardwaj RK, Gupta SK HPLC 0.52–52 mg/mL 0.029 mg/mL Liquid-liquid 102.30% Methanol, acetonitrile and an aqueous solution of diammonium hydrogen orthophosphate (50 mM) in the ratio of 4:1:5 Human 14
Yuan Y, Chen X, Zhong D LC-MS 0.10–50.0 ng/mL Protein precipitation 97.20 % Acetonitrile–water–formic acid (80:20:0.2, v/v/v) Human 18
Salunkhe N, Jadhav N, Bhinge S. UV-HPLC 0.5-25 μg mL−1 0.0081 μg mL–1 Protein precipitation 94.71 % 15 mM phosphate buffer (pH 4.7 adjusted with dilute ortho-phosphoric acid solution): methanol (40:60 v/v) Rabbit Plasma Proposed Method