Table 7 Comparison of the developed HPLC method and the reported methods for analysis of MLC in plasma samples

Miyamoto A

HPLC-UV

20 to 1000 ng mL⁻¹

Not performed

Solid phase extraction

92.80 %

50 mM phosphate buffer (pH 2.15, solvent A) and acetonitrile (solvent B) at a flow rate of 0.8 mL/min. Isocratic elution (solvent A:solvent B = 60:40)

Rat

1

Dasandi B, Shivaprakash, Saroj H, Bhat KM

HPLC

50 to 1500 ng mL⁻¹

10 ng mL⁻¹

Protein precipitation

More than 85%

Sodium acetate buffer (pH 3.3, 170 mmol):acetonitrile (62:38 v/v) mixture

Human

4

Bae JW, Kim MJ, Jang CG, Lee SY

HPLC

10–2400 ng/mL

–

Liquid-liquid extract

Maximum 86.7 %

Acetonitrile–20mM potassium hydrogen phosphate (40:60, v/v, pH 3.5)

Human

5

Hye YJ, Hye WL, Young HK, et al

LC–MS/MS

0.50–200 (ng/mL)

–

Liquid-liquid extraction

87.1 % maximum

Methanol:ammonium formate (15 mM, pH 3.0) (60:40, v/v)

Human

7

Pairis-Garcia MD, Johnson AK, Kukanich B, et al

HPLC-MS

–

0.005 μg/mL

Protein precipitation

–

A. 0.1% formic acid in water and B. 0.1% formic acid in an acetonitrile at a flow rate of 0.250 mL/min

Swine

10

Shukla M, Singh G, Sindhura BG, et al

HPLC

–

0.02

μg mL⁻¹

Protein precipitation

89.00–93.00 %.

65% water:acetic acid (99:1, v/v) and 35% acetonitrile

Human

12

Velpandian T, Jaiswal J, Bhardwaj RK, Gupta SK

HPLC

0.52–52 mg/mL

0.029 mg/mL

Liquid-liquid

102.30%

Methanol, acetonitrile and an aqueous solution of diammonium hydrogen orthophosphate (50 mM) in the ratio of 4:1:5

Human

14

Yuan Y, Chen X, Zhong D

LC-MS

0.10–50.0 ng/mL

–

Protein precipitation

97.20 %

Acetonitrile–water–formic acid (80:20:0.2, v/v/v)

Human

18

Salunkhe N, Jadhav N, Bhinge S.

UV-HPLC

0.5-25 μg mL⁻¹

0.0081 μg mL^–1

Protein precipitation

94.71 %

15 mM phosphate buffer (pH 4.7 adjusted with dilute ortho-phosphoric acid solution): methanol (40:60 v/v)

Rabbit Plasma

Proposed Method