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Table 1 Preliminary trial runs

From: Development and validation of stability-indicating RP-HPLC method for the simultaneous determination of ertugliflozin pidolate and metformin hydrochloride in bulk and tablets

Trial Column Mobile phase Observation
1 BDS C18
(250 mm × 4.6 mm, 5 μm)
Methanol:
0.1 % OPA
(40:60)
Only MET was eluted with less theoretical plates
2 BDS C18
(250 mm × 4.6 mm, 5 μm)
ACN: KH2PO4
(40:60)
Only MET was eluted
3 Agilent C18
(250 mm × 4.6 mm, 5 μm)
ACN: 0.1 % OPA
(60:40)
Peak symmetry of both the peaks was good but with increased retention time
4 Kromasil C18
(150 mm × 4.6 mm, 5 μm)
ACN: 0.1 % OPA
(50:50)
Peak symmetry was good but MET eluted at 1.6 min
5 Kromasil C18
(150 mm × 4.6 mm, 5 μm)
ACN: 0.1 % OPA
(65:35)
Peak symmetry was good with system suitability parameters in limits