Table 4 Robustness parameters
Parameter | Method condition | Mean peak Area ± SD | %RSD | Rt ± SD | %RSD | |
|---|---|---|---|---|---|---|
Original | Variation | |||||
Flow rate (ml/min) | 1.2 | 1.4 | 19,425.2 ± 91.61 | 0.472 | 3.45 ± 0.036 | 1.04 |
1.2 | ||||||
1.0 | ||||||
Mobile phase (ACN to pH 2.5 phosphate buffer) | 85:15 | 87:13 | 19,352.7 ± 79.95 | 0.41 | 3.43 ± 0.04 | 1.16 |
85:15 | ||||||
83:17 | ||||||