Exploration of in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic potentials with phytochemical screening of flowers of Brassica nigra

Future Journal of Pharmaceutical Sciences

Table 4 Effects of MCE and its fractions of B. nigra flowers on mice in the open and closed arm of the EPM

Sample	Doses (mg/kg)	Time spent in seconds (mean ± SEM)		Number of entries (mean ± SEM)
Sample	Doses (mg/kg)	Open arm	Closed arm	Open arm	Closed arm
Control	10	19.40 ± 2.64	254.00 ± 2.07	7.00 ± 0.71	28.33 ± 2.03
Standard	1	102.20 ± 3.71	170.00 ± 3.49	18.60 ± 0.51	7.33 ± 1.45
n-HSF	200	72.20 ± 2.71**	192.80 ± 2.35**	13.60 ± 0.81**	6.40 ± 0.51***
n-HSF	400	80.80 ± 1.46*	183.00 ± 2.43	14.80 ± 0.80	8.68 ± 2.11***
CSF	200	59.80 ± 4.53**	205.00 ± 1.87**	11.40 ± 0.68**	7.60 ± 0.51***
CSF	400	72.60 ± 2.01**	195.40 ± 1.75**	13.00 ± 0.71**	9.62 ± 0.61***
EASF	200	43.20 ± 1.43**	230.60 ± 1.75***	7.60 ± 0.51***	10.60 ± 0.51***
EASF	400	48.80 ± 1.46***	224.20 ± 1.88***	9.60 ± 0.60***	12.43 ± 0.60***
MCE	200	59.20 ± 0.97**	210.20 ± 1.85**	10.60 ± 0.51**	11.40 ± 0.68***
MCE	400	66.80 ± 1.50**	199.60 ± 2.50***	12.40 ± 0.60**	13.12 ± 0.71**
ASF	200	38.60 ± 0.93***	234.80 ± 2.42***	6.40 ± 0.51***	13.60 ± 0.81**
ASF	400	44.00 ± 2.10***	226.40 ± 0.67***	8.60 ± 2.10***	14.80 ± 0.80*

Each value represents the mean ± SEM (n = 5)
MCE methanol crude extract, EASF ethyl acetate soluble fraction, n-HSF n-hexane soluble fraction, CSF chloroform soluble fraction, ASF aqueous soluble fraction, SEM standard error mean
The inter group significance was analysed by one-way ANOVA using Dunnet’s t-test. P < 0.05*, P < 0.01** and P < 0.001*** indicates weak, moderate and high significance respectively