Exploration of in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic potentials with phytochemical screening of flowers of Brassica nigra

Future Journal of Pharmaceutical Sciences

Table 5 Effects of MCE and its fractions of B. nigra flowers on mice in hole board test

Sample	Doses (mg/kg)	Number of head dipping (300 s test) in hole board (mean ± SEM)
Control	10	36.40 ± 3.26
Standard	1	39.80 ± 2.22
n-HSF	200	36.40 ± 3.26
n-HSF	400	64.20 ± 1.77***
CSF	200	72.20 ± 2.71***
CSF	400	71.60 ± 2.20***
EASF	200	81.20 ± 2.60***
EASF	400	46.00 ± 1.41
MCE	200	57.00 ± 1.22**
MCE	400	63.60 ± 1.72***
ASF	200	75.20 ± 1.71**
ASF	400	45.00 ± 3.21

Each value represents the mean ± SEM (n = 5)
MCE methanol crude extract, EASF ethyl acetate soluble fraction, n-HSF n-hexane soluble fraction, CSF chloroform soluble fraction, ASF aqueous soluble fraction, SEM standard error mean
The inter group significance was analysed by one-way ANOVA using Dunnet’s t-test. P < 0.05*, P < 0.01** and P < 0.001*** indicates weak, moderate and high significance respectively