Table 5 Robustness of the method
Parameter changes | Retention time | Area ratio | Theoretical plates | Tailing factor | Resolutiona | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
IBU | CAF | IBU/IS | CAF/IS | IBU | CAF | IBU | CAF | IBU | CAF | ||
Initial temperature of the oven | 148 °C | 2.61 | 4.10 | 50.70 | 3.65 | 128475 | 125034 | 1.04 | 1.12 | 19.77 | 25.00 |
150 °C | 2.59 | 4.03 | 50.66 | 3.62 | 129940 | 125500 | 1.02 | 1.10 | 19.99 | 25.01 | |
152 °C | 2.53 | 4.00 | 50.59 | 3.60 | 130100 | 126500 | 1.01 | 1.09 | 20.00 | 25.55 | |
%RSD | 1.66 | 1.25 | 0.11 | 0.70 | – | – | – | ||||
Flow rate | 0.9 mL/min | 2.61 | 4.09 | 50.9 | 3.65 | 128888 | 122847 | 1.03 | 1.12 | 20.00 | 24.99 |
1 mL/min | 2.59 | 4.02 | 50.60 | 3.61 | 129925 | 125499 | 1.01 | 1.10 | 20.01 | 25.19 | |
1.1 mL/min | 2.52 | 4.01 | 50.50 | 3.58 | 130487 | 127374 | 1.00 | 1.09 | 20.09 | 25.25 | |
%RSD | 1.84 | 1.08 | 0.41 | 0.97 | – | – | – | ||||