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Table 4 Matrix effect and matrix factor for bexarotene at LQC level (MTR-BA-LC-MS/MS-05)

From: Development and validation of bexarote by bioanalytical methods using liquid chromatography-tandem mass spectroscopy (LC-MS/MS)

  Aqueous sample Spiked sample Matrix factor of analyte Matrix factor of IS IS normalized matrix factor Area ratio
Analyte area IS area Analyte area IS area Aqueous sample Spiked sample
MAT-6223-I 48,348 1,336,922 45,987 1,317,311 1.08 1.03 1.05 0.036 0.03
MAT-6224-I 42,001 1,271,968 42,437 1,277,296 0.99 1.00 0.99 0.033 0.03
MAT-6225-I 43,318 1,289,768 40,402 1,257,917 0.95 0.98 0.97 0.033 0.03
MAT-6220-I 37,838 1,240,867 42,187 1,239,881 0.99 0.97 1.02 0.030 0.03
MAT-6204-I 41,861 1,246,249 43,795 1,222,111 1.03 0.96 1.07 0.033 0.03
MAT-6205-I 42,559 1,278,563 41,373 1,221,142 0.97 0.96 1.01 0.033 0.03
MAT-C-0544-XII(H)    43,332 1,289,828 1.02 1.01 1.01   0.03
MAT-6188-IX (L)    42,395 1,282,621 0.99 1.00 0.99   0.03
MAT-6198-(X)-heparin plasma    43,054 1,277,420 1.01 1.00 1.01   0.03
Mean 42,654.16 1,277,389   1.003 0.99 1.01333 0.033 0.03
SD   0.037 0.023 0.030822  
%CV 3.76 2.42 3.04