Table 8 Intra-batch precision and accuracy of bexarotene

Calculated concentration (ng/mL) (MTR-BA-LC-MS/MS-19) PA–01

(06 Apr 2015)

1.1639

2.9471

25.8272

135.3234

277.1292

1.1622

2.8307

26.0325

128.5281

276.5622

1.0975

2.9013

25.3157

131.3295

272.8455

1.0866

2.8823

25.3351

132.7338

267.9314

1.1758

3.0348

26.1218

136.8898

264.0381

1.1043

2.9858

26.5157

133.2429

277.9904

Mean

1.13172

2.93033

25.85800

133.00792

272.74947

SD

0.039664

0.073957

0.469270

2.947768

5.662107

%CV

3.50

2.52

1.81

2.22

2.08

%nominal

107.78

105.41

102.33

101.06

103.62

Calculated concentration (ng/mL) (MTR-BA-LC-MS/MS-19) PA–02

(07 Apr 2015)

1.0709

2.7864

26.1883

134.5485

270.4602

1.0005

2.8122

25.8356

134.5475

274.3989

0.8813

2.8064

25.3888

138.3150

266.9063

1.0456

2.8642

25.3942

133.0021

272.3938

1.1056

2.9071

25.5199

132.3109

266.4461

1.0615

2.8626

25.6117

136.5209

273.7517

Mean

1.02757

2.83982

25.65642

134.87415

270.72617

SD

0.079461

0.045549

0.308551

2.228847

3.417745

%CV

7.73

1.60

1.20

1.65

1.26

%Nominal

97.86

102.15

101.53

102.48

102.85