Evaluation of chemo-preventive efficacy of Ficus religiosa latex extract by flow cytometry analysis and gene expression studies performed by RT-PCR in various cell lines

Future Journal of Pharmaceutical Sciences

Table 3 IC₅₀ (μg/ml) values of F. religiosa latex extracts on human cancer cell lines exhibiting best activity

S. no	Cell lines	Ficus religiosa latex extracts	IC₅₀ (μg/ml)
1.	MDA MB 231	Ethanol	50.86 ± 0.9*
		Methanol	145.5 ± 4.5
		Acetone	111.86 ± 1.56
		Ethyl acetate	107.25 ± 4.12
		Doxorubicin	0.82 ± 0.13
2.	IMR 32	Ethanol	4.8 ± 1.13*
		Methanol	146.01 ± 1.7
		Acetone	102.84 ± 0.9
		Ethyl acetate	39.24 ± 3.21*
		Doxorubicin	0.37 ± 0.05
3.	HCT 116	Ethanol	24.27 ± 0.66*
		Methanol	122.89 ± 4.01
		Acetone	120.71 ± 3.01
		Ethyl acetate	101.65 ± 1.6
		Doxorubicin	0.48 ± 0.3

Each value is the average of three analyses ± standard deviation of 3 separate experiments in three selected cancer cell lines.
*The IC₅₀ values of the extract in comparison with control drug among all the cell lines were statistically significant (p < 0.001; t test).