Table 24 Robustness study results of N-hexane

Flow rate (± 0.2 mL/min)

2.3

3.2

12.45

79584

1.0

2.5

3.4

11.31

78987

1.3

2.7

3.5

10.19

72142

1.1

Column oven temperature (± 5 °C)

35 °C

3.2

12.47

79658

1.1

40 °C

3.4

11.31

78987

1.3

45 °C

3.6

10.21

77415

1.2

Vial oven temperature (± 5 °C)

85 °C

3.0

11.30

79584

1.3

90 °C

3.4

11.31

78987

1.3

95 °C

3.3

11.32

80154

1.2