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Table 2 Summary of robustness studies

From: Stability indicating thin-layer chromatographic method for estimation of antidiabetic drug Remogliflozin etabonate

Method/parameter condition Normal conditions Normal Rf value Deliberate changes Rf ± S.D. (n = 3) Effect on drug spot
Mobile phase ratio (Methanol: ethyl acetate: toluene: ammonia, v/v/v) 2:4:4:0.1 0.61 2:4.5:3.5:0.1 0.64 ± 0.01 Compact band
2:3.5:4.5:0.1 0.59 ± 0.03 Compact band
Chamber saturation time (min) 30 25 0.62 ± 0.01 Compact band
35 0.63 ± 0.02 Compact band
Migration distance (mm) 80 85 0.61 ± 0.01 Compact band
75 0.62 ± 0.02 Compact band