Table 6 Robustness study
Sr. no. | Xylometazoline | Ipratropium bromide | Xylometazoline | Ipratropium bromide | |
|---|---|---|---|---|---|
Decrease in flow rate (0.9 mL/min) | Increase in flow rate (1.1 mL/min) | ||||
1 | % RSD of six standard inj. | 0.85% | 0.47% | 0.33 | 0.36 |
Tailing factor | 1.5 | 1.5 | 1.6 | 1.5 | |
Theoretical plates | 47107 | 8743 | 48581 | 8230 | |
Change in column oven temperature ±5 °C of 45 °C | |||||
2 | Decreases in column temperature (40 °C) | Increases in column temperature (50 °C) | |||
% RSD of six standard inj. | 0.48 | 0.28 | 0.29 | 0.24 | |
Tailing factor | 1.6 | 1.5 | 1.6 | 1.5 | |
Theoretical plates | 50569 | 6784 | 40259 | 6510 | |
Change in wavelength ± 3 nm of 210.0 nm | |||||
3 | Decrease in wavelength (207.0 nm) | Increase in wavelength (213.0 nm) | |||
% RSD of six standard inj. | 0.08 | 0.20 | 0.06 | 0.28 | |
Tailing factor | 1.4 | 1.6 | 1.4 | 1.6 | |
Theoretical plates | 50027 | 5214 | 50891 | 5323 | |