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Table 6 Robustness study

From: Development and validation of stability-indicating RP-HPLC method for the simultaneous estimation of xylometazoline hydrochloride and ipratropium bromide from nasal spray dosage form

Sr. no.   Xylometazoline Ipratropium bromide Xylometazoline Ipratropium bromide
   Decrease in flow rate (0.9 mL/min) Increase in flow rate (1.1 mL/min)
1 % RSD of six standard inj. 0.85% 0.47% 0.33 0.36
  Tailing factor 1.5 1.5 1.6 1.5
  Theoretical plates 47107 8743 48581 8230
  Change in column oven temperature ±5 °C of 45 °C
2   Decreases in column temperature (40 °C) Increases in column temperature (50 °C)
  % RSD of six standard inj. 0.48 0.28 0.29 0.24
  Tailing factor 1.6 1.5 1.6 1.5
  Theoretical plates 50569 6784 40259 6510
  Change in wavelength ± 3 nm of 210.0 nm
3   Decrease in wavelength (207.0 nm) Increase in wavelength (213.0 nm)
  % RSD of six standard inj. 0.08 0.20 0.06 0.28
  Tailing factor 1.4 1.6 1.4 1.6
  Theoretical plates 50027 5214 50891 5323