A rapid RP-HPLC method for the simultaneous estimation of Ivacaftor and Tezacaftor and in silico study of their metabolitic products

Future Journal of Pharmaceutical Sciences

Table 10 Robustness study of Tezacaftor and Ivacaftor in Mobile phase and Human plasma

S. no.	Parameters	Normal	Variation	%RSD in mobile phase		%RSD in human plasma
S. no.	Parameters	Normal	Variation	Tezacaftor	Ivacaftor	Tezacaftor	Ivacaftor
1	Wavelength	235 nm	225 nm	0.77	0.8	0.38	0.54
1	Wavelength	235 nm	245 nm	0.2	0.03	0.36	0.106
2	Temperature	30 ^°C	25 ^°C	1.035	1.7	0.24	0.44
2	Temperature	30 ^°C	35 ^°C	0.76	0.58	0.15	0.38
3	Flow Rate	1 mL/min	0.8 mL/min	0.64	0.13	0.298	0.22
3	Flow Rate	1 mL/min	1.2 mL/min	0.125	0.06	0.02	0.06