Table 4 Robustness for DEC and DEH assay
Parameter | Condition applied | DEC | DEH | ||||
|---|---|---|---|---|---|---|---|
ReA | SV | RSV | ReA | SV | RSV | ||
Methanol ratio (%) | 45 (opt) | 4258655 | 71394.7813 | 1.682 | 2184928 | 42212.8782 | 1.936 |
40 (varied) | 4308985 | 2220846 | |||||
50 (varied) | 4168097 | 2136719 | |||||
Flow stream (mL/min) | 1.0 (opt) | 4258655 | 77743.4253 | 1.833 | 2184928 | 30052.5910 | 1.381 |
0.9 (varied) | 4156414 | 2142693 | |||||
1.1 (varied) | 4308985 | 2200846 | |||||
pH value | 4.5 (opt) | 4311359 | 48299.1214 | 1.121 | 2204010 | 15598.0466 | 0.708 |
4.3 (varied) | 4355115 | 2215842 | |||||
4.7 (varied) | 4258655 | 2184928 | |||||
Detection (nm) | 265 (opt) | 4258655 | 76387.8855 | 1.794 | 2184928 | 39019.0224 | 1.788 |
263 (varied) | 4333890 | 2142622 | |||||
267 (varied) | 4181120 | 2220565 | |||||
Temperature (°C) | 25 (opt) | 4311359 | 48299.1214 | 1.121 | 2204010 | 15598.0466 | 0.708 |
23 (varied) | 4355115 | 2215842 | |||||
27 (varied) | 4258655 | 2184928 | |||||