Table 6 Analysis of robustness and ruggedness using apigenin solution

Mobile phase(± 2 v/v)

55:45

4.30

0.08

53:47

4.51

0.10

57:43

4.00

0.03

Flow rate(± 0.1 ml)

1 ml/min

4.33

0.08

0.9 ml/min

4.71

0.10

1.1 ml/min

3.86

0.09

Detection wavelength (± 2 nm)

267

4.24

0.13

269

4.23

0.11

Column oven temp(±1 °C)

35 °C

4.21

0.13

34 °C

4.22

0.15

36 °C

4.20

0.17

Column

Luna 5u(250X4.6)

4.33

0.08

Luna 5u(150X4.6)

3.84

0.09