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Table 6 Analysis of robustness and ruggedness using apigenin solution

From: A single robust stability-indicating RP-HPLC analytical tool for apigenin quantification in bulk powder and in nanoliposomes: a novel approach

Parameters Variations Time (min) %RSD
Mobile phase(± 2 v/v) 55:45 4.30 0.08
53:47 4.51 0.10
57:43 4.00 0.03
Flow rate(± 0.1 ml) 1 ml/min 4.33 0.08
0.9 ml/min 4.71 0.10
1.1 ml/min 3.86 0.09
Detection wavelength (± 2 nm) 267 4.24 0.13
269 4.23 0.11
Column oven temp(±1 °C) 35 °C 4.21 0.13
34 °C 4.22 0.15
36 °C 4.20 0.17
Column Luna 5u(250X4.6) 4.33 0.08
Luna 5u(150X4.6) 3.84 0.09
  1. RSD relative standard deviation