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Table 1 33 Box-Behnken full factorial design of DOE

From: Development and validation of RP-HPLC method for estimation of brexpiprazole in its bulk and tablet dosage form using Quality by Design approach

Runs

Factor 1

Factor 2

Factor 3

Response 1

Response 2

Response 3

A: ACN

B: pH

C: flow rate

R.T. (min)

Asymmetry

TP

1

65

2.50

1.0

4.22

1.34

3662

2

75

3.00

1.0

4.03

1.51

4067

3

75

2.50

0.8

5.04

1.37

3945

4

65

3.00

0.8

5.33

1.3

2986

5

65

2.00

0.8

5.37

1.43

3305

6

75

2.50

1.2

3.41

1.36

3377

7

65

3.00

1.2

3.54

1.45

3461

8

65

2.50

1.0

4.22

1.34

3662

9

55

3.00

1.0

4.61

1.02

1566

10

55

2.50

1.2

3.84

1.07

1598

11

55

2.00

1.0

4.83

0.93

1266

12

65

2.00

1.2

3.6

1.41

2753

13

65

2.50

1.0

4.23

1.34

3662

14

65

2.50

1.0

4.23

1.34

3662

15

55

2.50

0.8

5.66

0.99

1755

16

75

2.00

1.0

4.13

1.52

3246

17

65

2.50

1.0

4.22

1.34

3662