Table 2 Experimental runs for Box-Behnken design
Formulation | Factor A | Factor B | Factor C | Response 1 | Response 2 |
|---|---|---|---|---|---|
|  | Concentration of chitosan % w/v | Concentration of CMI % w/v | Concentration of drug % w/v | Particle size nm±SD | Entrapment efficiency % nm±SD |
1 | 0.2 (+ 1) | 0.08 (0) | 0.5 (+ 1) | 199.25±1.2 | 79.20±2.2 |
2 | 0.15(0) | 0.08 (0) | 0.3 (0) | 179.41±2.2 | 72.32±3.3 |
3 | 0.1 (−1) | 0.08 (0) | 0.5 (+ 1) | 160.36±3.0 | 59.36±4.5 |
4 | 0.1 (−1) | 0.06 (−1) | 0.3 (0) | 15,535±3.6 | 66.14±5.2 |
5 | 0.15 (0) | 0.1 (+ 1) | 0.5 (+ 1) | 188.85±4.5 | 64.25±5.0 |
6 | 0.1 (−1) | 0.08 (0) | 0.1 (−1) | 151.62±1.5 | 66.22±3.6 |
7 | 0.2 (+ 1) | 0.06 (−1) | 0.3 (0) | 208.55±1.8 | 86.50±1.4 |
8 | 0.2 (+ 1) | 0.1 (+ 1) | 0.3 (0) | 192.55±1.5 | 77.45±1.5 |
9 | 0.15 (0) | 0.1 (+ 1) | 0.1 (−1) | 191.27±2.0 | 71.48±2.0 |
10 | 0.1 (−1) | 0.1 (+ 1) | 0.3 (0) | 174.22±2.8 | 55.34±3.5 |
11 | 0.15 (0) | 0.06 (−1) | 0.5 (+ 1) | 193.32±4.2 | 73.38±1.8 |
12 | 0.15 (0) | 0.08 (0) | 0.3 (0) | 180.20±5.2 | 73.49±1.6 |
13 | 0.2 (+ 1) | 0.08 (0) | 0.1 (−1) | 184.18±2.5 | 88.58±1.0 |
14 | 0.15 (0) | 0.08 (0) | 0.3 (0) | 178.42±1.8 | 72.25±2.2 |
15 | 0.15 (0) | 0.06 (−1) | 0.1 (−1) | 179.34±3.6 | 82.54±2.6 |