Table 2 Chromatographic standardization of Quercetin in herbal medicines
Sl. no | Author name | Title of the work | Description of analysis |
|---|---|---|---|
1 | Sunita shailajan et al. | A comparative estimation of quercetin content from Cuscuta reflexa Roxbusing validated HPTLC and HPLC techniques | By HPLC method using 0.025 M NaH2PO4: ACN as mobile phase. The analysis was performed using C18 column (150 mm × 4.6 mm × 5 µm) peaks were recorded at 378 nm |
2 | AsmaˈaAi-Rifai et al. | Analysis of Quercetin and Kaempferol in an Alcoholic Extract of Convolvulus pilosellifolius using HPLC | HPLC method using isocratic mixture of methanol and water containing 0.1% v/v formic acid (80:20) using BETASIL C18 column (150 mm × 4.6 mm,3 µm) and peaks were found at 258 nm |
3 | Lee Fung Ang et al. | HPLC Method for Simultaneous Quantitative Detection of Quercetin and Curcuminoids in Traditional Chinese Medicines | Quantitative detection of quercetin and curcuminoids (dimethoxy curcumin, bis demethoxy curcumin, curcumin) in traditional Chinese medicines by HPLC using thermo ersil Gold column (250 mm × 4.6 mm ID.:5 µm) and a C-18 cartridge guard column (12.5 mm × 4.6mmID.:5 mm) with a mobile phase system of Acetonitrile and 2%v/v acetic acid (40:60) at the detection wavelength 370 nm |
4 | Ujjwala supe et al. | Preliminary Phytochemical Analysis and Quantitative Analysis of Quercetin by HPLC of Momordica Charantia | HPLC method performed using Merck C-18 Bondapack which is maintained at a temperature of 27℃ and methanol:ACN:water (60:20:20v/v) were used as mobile phase. At 260 and 262 nm wavelength the investigation carried out for flavonoid, phenols and Quercetin [14] |
5 | A. Srinivasa Rao et al. | Simultaneous determination of phenolic compounds in Catharanthus roseus leaves by HPLC method | HPLC method using Athena C18 column and phosphate buffer (pH = 5.8) and ACN as mobile phase 55:45 Ratio and maximum absorbance were measured at 254 nm. The retention time of Rutin, Quercetin, Kaempferol was found 2.403, 6.143, 8.903 respectively |
6 | Deepak Mundkinajeddu et al. | Development and Validation of High Performance Liquid Chromatography Method for Simultaneous Estimation of Flavonoid Glycosides in Withania somnifera Aerial Parts | HPLC method by using Phenomenex Luna C18 column (5µ, 250 × 4.6 mm) and the mobile system consist of potassium dihydrogen orthophosphate (0.136 g) dissolved in 900 ml of HPLC grade water to that 0.5 ml of orthophosphoric acid added volume made up to 1000 ml, for the estimation of 3 flavonoid glycosides that are quercetin-3-orobinoside-7-O-glucoside(1), quercetin- 3-O-rutinoside-7-O-glucoside (2), kaempferol 3-O-robinobioside-7-O-glucoside(3) |
7 | Aline Augusti Boligon et al. | Development and Validation of an HPLC–DAD Analysis for Flavonoids in the gel of Scutia buxifolia | They have validated the HPLC using C18 column and mixture of Acetonitrile: water (70:30, v/v) as a mobile phase at 356 nm of maximum absorbance wavelength the quercetin and rutin are quantified |
8 | Gomathy Subramanian et al. | Development and Validation of HPLC Method for the Simultaneous Estimation of Quercetin and Rutin in Aganosma Dichotoma [Roth] K. Schum | For HPLC method separation is achieved using C18 column (150 × 4.6 mm) and mobile phase containing acetonitrile: ammonium acetate (40:60v/v) at the flow rate 1 ml/min.the analysis monitored at 259 nm |
9 | Haritha Krishna prasad et al. | Method Development and Validation for the Simultaneous Estimation of Resveratrol and Quercetin in Bulk and Pharmaceutical Dosage Form by RP-HPLC | RP-HPLC method for the estimation is performed by using Sunfire C18 (150 × 3.0nmI.D5µm particle size) column having Rheodyne injector using Methanol:Water:Formic acid: Triethylamine in the ratio 10:70:15:5 as mobile phase at the wavelength 277 nm.resveratrol and Quercetin eluted at retention time 1.24 and 2.14 respectively |
10 | Vishal Sharad Chaudhari et al. | Analytical method development and validation of reverse-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous quantifications of quercetin and piperine in dual-drug loaded nanostructured lipid carriers | RP-HPLC method for The estimation was carried out by using Hypersil gold C-18 column (150 mm × 4.6 mm, 5 µm particle size ODS) and mixture of acetonitrile and HPLC grade water (pH 2.6 adjusted with 2%v/v glacial acetic acid) as a mobile phasewavelength 346 nm Quercetin and Piperine is eluted at retention time 2.80 min and 10.36 min respectively |
11 | Ashok Kumar et al. | Estimation of Gallic acid, Rutin and Quercetin in Termenelia chebulaby HPTLC | HPTLC method by using precoated silica gel GF254 as stationary phase and Toluene: acetone: glacial acetic acid (3:2:1 v/v/v/v/v) as mobile phase for of tannins, and ethyl acetate: dichloromethane: formic acid: glacial acetic acid: water (10:2.5:1:1:0.1v/v/v/v/v) the quantification is carried out for Rutin and Quercetin at 366 nm and for gallic acid at 254 nm densitometrically and Rf value of gallic acid, rutin, and quercetin are 0.30, 0.13, 0.93 respectively |
12 | Sachin U. Rakesh et al. | HPTLC Method For Quantitative determination of Quercetin in Hydroalcoholic Extract Of Dried Flower of Nymphaea Stellata Willd | In HPTLC methodthe detection and quantification were carried out by silica gel 60 F254 plates and Toluene: ethyl acetate: formic acid in the ratio 5:4:0.2 (v/v/v) as mobile phase. At 380 nm a sharp and well-defined peak found at Rf = 0.29 |
13 | Ansul Shakya et al. | A Rapid High-Performance Thin-Layer Chromatographic Method to Estimate Quercetin in Benin casahispida (Thunb.) Cogn. Fruit Pulp | HPTLC for the estimation using alumina plates with silica gel 60 F254 were used with Toluene: ethyl acetate: formic acid (5:4:0.2v/v) as mobile phase at absorbance wavelength 262nmthe Rf value of Quercetin found Rf = 0.392 |
14 | Barik Laxmi Dhar et al. | HPTLC method for Quantitative estimation of Quercetin in a polyherbal compound for urolithiasis | By HPTLC method extract chromatogrammed on silica gel 60F254 aluminium plates and mixture of chloroform: methanol: formic acid used as mobile phase in the ratio 7.5:1.5:1 v/v/v. The Rf value was 0.51 analysed at wavelength of 254 nm |
15 | Bindu A R et al. | High Performance Thin Layer Chromatographic Method for Quantitative Determination of Quercetin in Tender Leaves of Psidium guajava | HPTLC method caaried out on silica gel 60F254 TLC plates using toluene: acetone: formic acid (30:10:5) as mobile phase. The detection and quantification done at 364 nm and Rf value of acetone extract was 0.45 |
16 | Vaidevi Sethuram et al. | Combinatorial analysis of quercetin and resveratrol by HPTLC in Sesbania grandiflora /phyto based nanoformulations | By HPTLC method In this investigation separation achieved by using mobile phase system of toluene: chloroform: ethyl acetate: formic acid (3:2:4.9:0.1% v/v). The densitometric scanning at 280 nm was performed and Rf value was 0.40 (Quercetin) and 0.50(Resveratrol) |
17 | Mangesh S. Kharate et al. | Estimation of Quercetin from Crude Leaf Extract, Mimusops Elengi L. By HPTLC | The estimation of Quercetin carried out on precoated silica gel plates F 254 (10 cm × 10 cm) and mixture of toluene: ethyl acetate: formic acid in the ratio 5:4:1 was used as mobile phase. The detection and quantification done at 365 nm |
18 | V Patil et al. | Recent progress in simultaneous estimation of rutin, quercetin and liquiritin in Cocculus hirsutus by HPTLC | The simultaneous estimation carried on silica gel 60F254 and mixture of n-butanol: acetic acid: water: formic acid (7:1:1:0.25) as mobile phase system. The Rf value for Rutin, Quercetin and Liquiritin was 0.047 ± 0.03, 0.063 ± 0.03 and 0.82 ± 0.02 respectivelyat the wavelength 254 nm |
19 | Omi Laila et al. | Development and Validation of HPTLC Method for Simultaneous Estimation of Diosgenin and Quercetin in Fenugreek Seeds (Trigonella foenum-graceum) | HPTLC method for validation carried out using precoated aluminium plates with silica gel G60F254 and mixture of toluene: ethyl acetate: formic acid (5:4:1 v/v/v) as mobile phase. The scanning of plates at 275 nm and the Rf value was 0.69 ± 0.02 (Diosgenin) and 0.57 ± 0.02(Quercetin) |
20 | A. Srinivas Rao et al. | Simultaneous estimation of quercetin and rutin in ethanolic extract of Melia azedarach. Linn leaves by HPTLC method | HPTLC method forthe analysis is carried out with precoated silica 60F254 as stationary phase and mixture of toluene: ethyl acetate: methanol (5:3:2) as mobile phase. At 254 nm the Rf values of Quercetin and Rutin was 0.65 and 0.15 respectively |
21 | Gundu Sindhu Chakraborty et al. | Determination of Quercetin by HPTLC in “Calendula Officinalis” Extract | HPTLC method using precoated aluminium plates with silica gel 60GF as stationary phase and chloroform: methanol (9.5:0.5) mixture as mobile phase solvent system. The Rf value was 0.43 and scanning for estimation was carried out at 366 nm wavelength |
22 | Md. Sarfaraj Hussain et al. | Validation of the method for the simultaneous estimation of bioactive marker gallic acid and quercetin in Abutilon indicum by HPTLC | The analysis performed on aluminium foil-backed silica gel 60F254 HPTLC plates using solvent system of toluene: ethyl acetate: formic acid (5:4:1 v/v) as mobile phase. At absorbance wavelength 270 nm and Rf value was 0.31 (gallic acid), 0.50 (Quercetin) |
23 | Jinan Hussain et al. | Qualitative and quantitative comparison of rutin, quercetin and gallic acid concentrations in Syrian Capparis spinosa. L Leaves | The analysis performed on silica gel 60F254 and mixture of ethyl acetate: glacial acetic acid: formic acid: distilled water (100:11:11:25) as mobile phase. At the scanning wavelength of 366 nm the Rf value obtained are 0.39 (Rutin), 0.79 (Quercetin) and at 254 nm the Rf value was 0.81 (gallic acid) |
24 | Abhay Prakash Mishra et al. | A Developed and Validated High-Performance Thin-Layer Chromatographic Method for the Quantitative Determination of Quercetin in Satyrium nepalense Tubers | HPTLC analysis carried with silica gel 60F254 as stationary phase using toluene: ethyl acetate: formic acid (7:5:1 v/v) as solvent system at 366 nm |
25 | Shiv K Yadav et al. | Development and validation of a HPTLC method for simultaneous estimation of quercetin, Chlorogenic acid and trigonelline in polyherbal antibacterial formulation | HPTLC analysis with aluminium plates 60F254 using solvent system chloroform: ethyl acetate:methanol: formic acid (5:3:1.5:0.5v/v/v/v) as mobile phase. The well separated spots were obtained with Rf values of 0.13, 0.24, 0.62 for trigonelline, chlorogenic acid, and quercetin respectively |
26 | Hiteksha Panchal et al. | Development of Validated High-performance Thin-layer Chromatography Method for Simultaneous Determination of Quercetin and Kaempferol in Thespesia populnea | Analysis performed On aluminium plates precoated with silica gel 60F254 using toluene: ethyl acetate: formic acid (6:4:0.3 v/v/v). At the scanning absorbance wavelength 366 nm, the Rf value obtained are 0.39 for Quercetin and 0.50 for kaempferol |
27 | Khan Dureshahwar et al. | Quantification of Quercetin Obtained from Allium cepa Lam. Leaves and its Effects on Streptozotocin-induced Diabetic Neuropathy | The quantification carried out with precoated silica gel GF254 plates and using mobile phase system toluene: ethyl acetate: formic acid (5:4:1) and the scanning absorbance at 254 nm |
28 | Supriya S. Jirge et al. | Simultaneous Estimation of Kaempferol, Rutin, and Quercetin in Various Plant Products and Different Dosage Forms of Bhuiamla and Amla | The analysis performed with precoated silica gel aluminium plates 60F254(20 cm × 10 cm with 250 µm thickness) and mixture of chloroform:methanol: formic acid (8.2:1.5:1) used as mobile phase and at 254 nm the Rf value of kaempferol-0.69, quercetin-0.53, and rutin-0.12 are reported |
29 | Shikar Verma et al. | HPTLC Analysis for the Simultaneous Quantification of Gallic Acid, Vanillic Acid, Protocatechuic Acid, and Quercetin in the Methanolic Fraction of Limonia acidissima L. Fruits | HPTLC method using silica gel 60F254 plates. The mixture of solvents toluene:ethyl acetate: formic acid (5:4:1) used as mobile phase and the detection carried out at 254 nm and reported Rf values are 0.3 ± 0.00 (gallic acid), 0.47 ± 0.005 (vanillic acid), 0.37 ± 0.00 (protocatechuic acid), 0.42 ± 0.005 (quercetin) |
30 | Snehal B. Bhandare et al. | Simultaneous Quantification of Kaempferol and Quercetin in Medicinal Plants using HPTLC | HPTLC method using toluene: acetone: formic acid in the ratio 7:3:0.25 as mobile phaseon RP-18 F254 plates. The Rf value for Kaempferol and Quercetin were found 0.46 and 0.39 respectively at the detection wavelength 254 nm |
31 | Shikar Verma et al. | Gas Chromatographic–Mass Spectrometric Profile of Non-Polar Fraction and High-Performance Thin-Layer Chromatographic Analysis of Methanolic Fraction with Simultaneous Quantifications of Proto catechuic Acid and Quercetin in Carissa carandas L. Fruits | HPTLC analysis using precoated plates with silica gel 60F254 with solvent system toluene: ethyl acetate: formic acid (6:3:1, v/v). The Rf value obtained as 0.57 and 0.61 for protocatechuic acid and quercetin respectively at the maximum absorbance wavelength 310 nm |
32 | Nadeem A. Siddique et al. | Simultaneous Quantification of Umbelliferone and Quercetin in Polyherbal Formulations of Aegle Marmelos by HPTLC | Analysis performed using precoated aluminium plates with silica gel 60F-254 as stationary phase. The solvent system consists of toluene: ethyl acetate: formic acid (6:4:1v/v/v) as mobile phase.The Rf value was reported as 0.66 and 0.68 for umbelliferone and quercetin at 300 nm |
33 | Pushpendra kumar et al. | Simultaneous Quantification of Quercetin and Syringic Acid in Methanolic Extract of Leucas lavandulifolia by using Validated HPTLCDensitometric Method | The analysis carried out on Silica gel 60F254 plate using Toluene: ethylacetate: formic acid (7:2.5:0.5 v/v) as mobile phase. The Rf value for quercetin and syringic acid was found to be 0.32 and 0.41 at the detection wavelength 275–370 nm |
34 | Thafshila Aafrin Am et al. | Determination qf Quercetin by High Performance Thin Layer Chromatography Method in Achyranthes Aspera (L.) Plant Extract | The determination carried out on silica gel 60F254 plate as stationary phase and mixture of toluene: ethyl acetate: formic acid in the ratio of 5:4:1. The Rf value 0.60 is detected at 254 nm |
35 | Avijeet Jain et al. | Simultaneous estimation of quercetin and rutin in Tephrosia purpurea Pers by high performance thin layer chromatography | The estimation carried out on precoated silica gel RP-18 F254 were used as stationary phase and the solvent system methanol: water: formic acid (40:57:3v/v/v) as mobile phase. At the detection wavelength 254nmthe Rf value was 0.07(quercetin) and 0.17 (rutin) |
36 | Girme AS et al. | Method development, optimization and validation of RP-UFLC method for bioactive flavonoids from Cassia auriculata | The analysis is carried out on acquity C18 column with stepwise gradient elution was carried out with 0.1% formic acid in water and methanol at a flow rate of 0.350 ml/min. The maximum absorbance at 350 nm wavelength was detected for quercetin QUE-3-O-rutinoside. The retention time was found at 3.95 and 5.37 for quercetin-3-O-rutinoside and quercetin respectively |
37 | Shanmugam R et al. | Development and Validation of a RP- UFLC Method for Simultaneous Estimation of Quercetin and Rutin | The analysis performed on reverse phase System C8 column (25 × 4.6 mm). the mobile phase consists of mixture of potassium dihydrogen ortho phosphate and acetonitrile the ratio of 70:30. The detection was done at 230 nm and the retention time was 7.4 and 2.8 min for quercetin and rutin respectively |
38 | Khaled Elgendy et al. | Analysis of Total Flavonoids in Herbal Drugs Expressed as Quercetin by Reversed Phase-UHPLC Method | The analysis done on column Phenomenex and methanol and 0.5% phosphoric acid (50:50v/v) as mobile phase with flow rate of 1.3 ml/min. Linearity of the method is established over the concentration range of 240-960 µg/ml for quercetin and retention time were found 7.8 min |
39 | Maric santos et al. | UPLC-MS for Identification of Quercetin Derivatives in Cuphea glutinosa Cham. and Schltdl (Lythraceae) and Evaluation of Antifungal Potential | The analysis performed on fast C18 column analytical column shim pack XR-ODS column (50 × 2 mm, 2.1 µm). the mobile phase consisted of mixture of acetonitrile: methanol (4:1v/v) as solvent A and water contains 0.1% formic acid as solvent B. the mass spectra was recorded by full scan mode in the range of m/z 200–800 |