Table 1 Rf values of Trigonella foenum-graecum seeds, coded ayurvedic formulation, and Diosgenin reference standard
Wavelength | Track 1: Trigonella foenum-graecum | Track 2: Hydrolysed Trigonella foenum-graecum | Track 3: Diosgenin | Track 4: Hydrolysed coded formulation extract | Track 5: Coded formulation extract | |||||
|---|---|---|---|---|---|---|---|---|---|---|
UV 254 nm | Rf | Colour | Rf | Colour | Rf | Colour | Rf | Colour | Rf | Colour |
– | – | – | – | – | – | 0.04 | Green | 0.05 | Green | |
– | – | 0.08 | Green | – | – | 0.07 | Green | – | – | |
– | – | – | – | – | – | 0.12 | Green | 0.13 | Green | |
– | – | 0.18 | Green | – | – | 0.19 | Green | – | – | |
– | – | – | – | – | – | 0.24 | Green | 0.24 | Green | |
– | – | 0.32 | Green | – | – | 0.33 | Green | 0.30 | Green | |
– | – | – | – | – | – | 0.37 | Green | 0.36 | Green | |
– | – | 0.41 | Green | – | – | – | – | 0.43 | Green | |
– | – | – | – | – | – | 0.64 | Green | – | – | |
– | – | 0.80 | Green | – | – | 0.78 | Green | – | – | |
– | – | – | – | – | – | 0.84 | Green | – | – | |
UV 366 nm | – | – | 0.04 | Pale blue | – | – | 0.04 | Pale blue | 0.04 | Pale blue |
– | – | 0.07 | Dark blue | – | – | – | – | – | – | |
0.09 | Blue | 0.11 | Purple | 0.11 | Purple | |||||
– | – | 0.22 | Blue | – | – | 0.24 | Purple | 0.25 | Red | |
– | – | – | – | – | – | 0.27 | Purple | 0.27 | Purple | |
– | – | 0.34 | Blue | – | – | 0.37 | Yellow | 0.37 | Yellow | |
– | – | – | – | – | – | – | – | 0.41 | Red | |
– | – | – | – | – | – | 0.45 | Blue | 0.45 | Blue | |
– | – | 0.52 | Blue | – | – | – | – | 0.51 | Purple | |
– | – | – | – | – | – | – | – | 0.56 | Red | |
– | – | – | – | – | – | 0.76 | Yellow | 0.76 | Yellow | |
Derivatized with anisaldehyde sulphuric acid reagent | 0.04 | Grey | 0.07 | Grey | – | – | – | – | 0.04 | Grey |
0.12 | Grey | 0.11 | Grey | – | – | – | – | 0.09 | Grey | |
– | – | 0.15 | Grey | – | – | – | – | 0.17 | Grey | |
– | – | 0.20 | Grey | – | – | 0.20 | Yellow | – | – | |
0.22 | Grey | 0.22 | Green | 0.22 | Green | 0.22 | Green | 0.22 | Grey | |
0.34 | Grey | 0.34 | Grey | – | – | 0.34 | Grey | 0.32 | Grey | |
– | – | – | – | – | – | 0.37 | Grey | – | – | |
0.41 | Grey | 0.41 | Grey | – | – | 0.41 | Grey | 0.41 | Grey | |