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Table 1 Rf values of Trigonella foenum-graecum seeds, coded ayurvedic formulation, and Diosgenin reference standard

From: Qualitative and quantitative estimation of Diosgenin in coded ayurvedic formulation and its ingredient Trigonella foenum-graecum Linn. seeds used in diabetics

Wavelength Track 1: Trigonella foenum-graecum Track 2: Hydrolysed Trigonella foenum-graecum Track 3: Diosgenin Track 4: Hydrolysed coded formulation extract Track 5: Coded formulation extract
UV 254 nm Rf Colour Rf Colour Rf Colour Rf Colour Rf Colour
0.04 Green 0.05 Green
0.08 Green 0.07 Green
0.12 Green 0.13 Green
0.18 Green 0.19 Green
0.24 Green 0.24 Green
0.32 Green 0.33 Green 0.30 Green
0.37 Green 0.36 Green
0.41 Green 0.43 Green
0.64 Green
0.80 Green 0.78 Green
0.84 Green
UV 366 nm 0.04 Pale blue 0.04 Pale blue 0.04 Pale blue
0.07 Dark blue
   0.09 Blue    0.11 Purple 0.11 Purple
0.22 Blue 0.24 Purple 0.25 Red
  0.27 Purple 0.27 Purple
0.34 Blue 0.37 Yellow 0.37 Yellow
0.41 Red
0.45 Blue 0.45 Blue
0.52 Blue 0.51 Purple
0.56 Red
0.76 Yellow 0.76 Yellow
Derivatized with anisaldehyde sulphuric acid reagent 0.04 Grey 0.07 Grey 0.04 Grey
0.12 Grey 0.11 Grey 0.09 Grey
0.15 Grey 0.17 Grey
0.20 Grey 0.20 Yellow
0.22 Grey 0.22 Green 0.22 Green 0.22 Green 0.22 Grey
0.34 Grey 0.34 Grey 0.34 Grey 0.32 Grey
0.37 Grey
0.41 Grey 0.41 Grey 0.41 Grey 0.41 Grey