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Table 1 Chromatographic conditions

From: Impurity profiling method development and validation of metformin hydrochloride and teneligliptin hydrobromide hydrate in their combination tablet dosage form by using RP-HPLC with UV/PDA detector

HPLC parameters Conditions
HPLC column BDS Hypersil C18, 250 × 4.5 mm, 5µ
Column temperature 35 °C
Sample cooler temperature Ambient
Flow rate 1 mL/min
Injection volume 20 µL
Wavelength 210 nm