From: Impurity profiling method development and validation of metformin hydrochloride and teneligliptin hydrobromide hydrate in their combination tablet dosage form by using RP-HPLC with UV/PDA detector
HPLC parameters
Conditions
HPLC column
BDS Hypersil C18, 250 × 4.5 mm, 5µ
Column temperature
35 °C
Sample cooler temperature
Ambient
Flow rate
1 mL/min
Injection volume
20 µL
Wavelength
210 nm