Table 1 Optimized chromatographic conditions

Mobile phase

Methanol: acetonitrile: water (50: 30: 20, by volume)

Column

Waters Acquity C₁₈ (50 mm × 2.1 mm ID, 1.8 µm)

Flow rate

0.5 mL/min

Column temperature

25 ± 2° C

Wavelength

260.00 nm (UV Detector)

Injection volume

10 µL

Elution

Isocratic mode

Run time

5 min

Retention time

 Bempedoic acid

1.827 min

 Ezetimibe

3.577 min