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Table 1 Optimized chromatographic conditions

From: Stability indicating RP-UPLC method for simultaneous quantification of bempedoic acid and ezetimibe in bulk and pharmaceutical formulations

Parameter Optimized condition
Mobile phase Methanol: acetonitrile: water (50: 30: 20, by volume)
Column Waters Acquity C18 (50 mm × 2.1 mm ID, 1.8 µm)
Flow rate 0.5 mL/min
Column temperature 25 ± 2° C
Wavelength 260.00 nm (UV Detector)
Injection volume 10 µL
Elution Isocratic mode
Run time 5 min
Retention time
 Bempedoic acid 1.827 min
 Ezetimibe 3.577 min