Table 5 Validation and stability parameters of the chemometrics optimized RP-HPLC method
Parameters | Metsulfuron-methyl | Chlorantraniliprole | Chlorimuron-ethyl |
|---|---|---|---|
Linearity correlation coefficient | Slope = 2966.9 Intercept = 13,062 (R2) = 0.999 | Slope = 62,403 Intercept = 13,062 (R2) = 0.999 | Slope = 3513.5 Intercept = 13,062 (R2) = 0.999 |
Precision | Mean = 934,911.8 SD = 7337.7 %RSD = 0.8 | Mean = 743,186.5 SD = 2511.5 %RSD = 0.3 | Mean = 1,107,995.2 SD = 8554.2 %RSD = 0.8 |
Intermediate precision | Mean = 960,417.7 SD = 8148.4 %RSD = 0.8 | Mean = 934,911.8 SD = 3655.2 %RSD = 0.5 | Mean = 934,911.8 SD = 3722.7 %RSD = 0.3 |
Accuracy (Mean recovery) | 99.95 | 99.86 | 100.27 |
LOD (S/N ratio) | 3.00 | 2.96 | 3.02 |
LOQ (S/N ratio) | 9.98 | 10.02 | 10.00 |
Acid degradation (%) | 3.26 | 2.50 | 10.89 |
Base degradation (%) | 1.58 | 1.05 | 9.99 |
Oxidative degradation (%) | 3.83 | 5.19 | 10.52 |
Reduction (%) | 1.71 | 3.85 | 8.21 |