Table 3 Robustness study

Change in flow rate

Area*

Area*

Area*

Area*

0.9 mL

59,662

345,373

428,300

36,922

1 mL

62,377

343,268

410,983

37,868

1.1 mL

61,702

330,567

403,890

36,036

Organic composition in mobile phase

10%less

60,835

334,251

418,302

36,554

Actual

62,377

343,268

410,983

37,868

10% more

61,473

319,463

420,831

37,831

Detection wavelength

252 nm

59,870

325,549

408,822

37,402

254 nm

62,377

343,268

410,983

37,868

256 nm

61,352

338,546

412,297

36,451

% RSD

–

1.64

1.85

1.89

1.32