Table 1 DoE experiment for method optimization
Factors | Responses | |||||
|---|---|---|---|---|---|---|
Wavelength | pH of phosphate buffer | Flow | Organic ratio (MeOH) | Retention time (NAP) | Retention time (ESP) | Tailing factor (NAP) |
254 | 7 | 0.5 | 40 | 44.5 | 63.4 | 1.37 |
6 | 0.5 | 45.9 | 65.1 | 1.41 | ||
7 | 1.5 | 12.9 | 23.5 | 1.24 | ||
6 | 1.5 | 14.1 | 24.3 | 1.31 | ||
300 | 7 | 0.5 | 44.5 | 63.4 | 1.39 | |
6 | 0.5 | 45.9 | 65.1 | 1.46 | ||
7 | 1.5 | 12.9 | 23.5 | 1.29 | ||
6 | 1.5 | 14.1 | 24.3 | 1.38 | ||
254 | 7 | 0.5 | 50 | 11.5 | 21.0 | 1.20 |
6 | 0.5 | 11.9 | 22.9 | 0.98 | ||
6 | 1.5 | 5.9 | 8.8 | 0.99 | ||
7 | 1.5 | 6.1 | 9.1 | 1.28 | ||
300 | 7 | 0.5 | 11.5 | 21.0 | 1.31 | |
6 | 0.5 | 11.9 | 22.9 | 1.10 | ||
6 | 1.5 | 5.9 | 8.8 | 0.90 | ||
7 | 1.5 | 6.1 | 9.1 | 1.25 | ||