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Table 2 Determination of total phenolic, flavonoid content, in vitro antioxidant activity and corresponding IC50 value of the ethanolic extract of L. rugosa

From: Ameliorative effect of ethanolic extract of Limnophila rugosa (Scrophulariaceae) in paracetamol- and carbon tetrachloride-induced hepatotoxicity in rats

Plant extracts and standard

Total phenolic content (mg GAE/g)

Total flavonoid content (mg QE/g)

In vitro antioxidant

DPPH %

Hydrogen peroxide %

Nitric oxide %

Ascorbic acid

83.45 ± 0.51

75.81 ± 0.46

86.23 ± 0.63

IC50 (μg/ml)

57.12 ± 0.17

47.29 ± 0.72

49.13 ± 0.33

L. rugosa

61.23 ± 1.31

53.11 ± 0.23

55.19 ± 0.45*

56.23 ± 0.81*

65.23 ± 0.23*

IC50 (μg/ml)

69.19 ± 0.58

102.23 ± 0.25

54.12 ± 0.23

  1. GAE: gallic acid equivalent, QE: quercetin equivalent
  2. Values are expressed as mean ± SEM, n = 3 and were estimated by one-way ANOVA followed by Dunnet test. *p ˂ 0.05 considered as significant