Validation of the HPLC–PDA method for detection of eluxadoline and rifaximin in rat plasma and application in a pharmacokinetic study

Future Journal of Pharmaceutical Sciences

Table 1 Intra- and Inter-day accuracy and precision results for ELX and RFX

Analytes	Concentration level (ng mL⁻¹)	Intra-day (n = 6)			Inter-day (n = 18)
Analytes	Concentration level (ng mL⁻¹)	Mean found conc. (ng mL⁻¹)	CV (%)	Accuracy (%)	Mean found conc. (ng mL⁻¹)	CV (%)	Accuracy (%)
ELX	LLOQ (5.0)	5.02 ± 0.023	0.465	99.17	5.04 ± 0.036	0.717	99.6
	LQC (50.0)	50.19 ± 0.207	0.413	99.65	50.43 ± 0.50	1.00	100.13
	MQC (100.0)	99.89 ± 0.134	0.134	99.72	99.78 ± 0.143	0.144	99.6
	HQC (150.0)	149.91 ± 0.311	0.207	99.68	149.94 ± 0.637	0.425	99.71
RFX	LLOQ (10.0)	10.19 ± 0.138	1.358	99.42	10.18 ± 0.12	1.186	99.39
	LQC (100.0)	100.36 ± 0.148	0.147	100.2	100.42 ± 0.131	0.130	100.2
	MQC (200.0)	200.21 ± 0.077	0.038	100.0	200.23 ± 0.143	0.071	100.0
	HQC (300.0)	302.16 ± 2.457	0.813	100.16	300.16 ± 2.157	0.714	100.61