Skip to main content

Table 4 The stability study of ELX and RFX under storage condition in rat plasma

From: Validation of the HPLC–PDA method for detection of eluxadoline and rifaximin in rat plasma and application in a pharmacokinetic study

Storage condition Conc. level ELX RFX
Concentration (ng mL−1) CV (%) Accuracy (%) Concentration (ng mL−1) CV (%) Accuracy (%)
Bench top (6 h at RT) LQC 50.0 0.41 97.0 100.15 0.14 98.3
HQC 150.0 0.1 93.7 300.3 0.04 94.6
Autosampler (24 h at 10 °C) LQC 50.0 0.54 96.7 100.15 0.07 98.5
HQC 150.0 0.09 93.7 300.3 0.04 94.7
Three freeze–thaw cycles (− 80 °C to RT) LQC 50.0 0.39 96.6 100.15 0.32 98.4
HQC 150.0 0.1 93.7 300.3 0.08 94.7
Long term (28 days at -80 °C) LQC 50.0 0.43 81.7 100.15 1.03 84.3
HQC 150.0 0.2 74.3 300.3 0.57 74.9
Dry extract (18 h at 10 °C) LQC 50.0 0.61 97.0 100.15 1.92 100.9
HQC 150.0 0.98 93.6 300.3 0.62 95.9