Validation of the HPLC–PDA method for detection of eluxadoline and rifaximin in rat plasma and application in a pharmacokinetic study

Future Journal of Pharmaceutical Sciences

Table 4 The stability study of ELX and RFX under storage condition in rat plasma

Storage condition	Conc. level	ELX			RFX
Storage condition	Conc. level	Concentration (ng mL⁻¹)	CV (%)	Accuracy (%)	Concentration (ng mL⁻¹)	CV (%)	Accuracy (%)
Bench top (6 h at RT)	LQC	50.0	0.41	97.0	100.15	0.14	98.3
Bench top (6 h at RT)	HQC	150.0	0.1	93.7	300.3	0.04	94.6
Autosampler (24 h at 10 °C)	LQC	50.0	0.54	96.7	100.15	0.07	98.5
Autosampler (24 h at 10 °C)	HQC	150.0	0.09	93.7	300.3	0.04	94.7
Three freeze–thaw cycles (− 80 °C to RT)	LQC	50.0	0.39	96.6	100.15	0.32	98.4
Three freeze–thaw cycles (− 80 °C to RT)	HQC	150.0	0.1	93.7	300.3	0.08	94.7
Long term (28 days at -80 °C)	LQC	50.0	0.43	81.7	100.15	1.03	84.3
Long term (28 days at -80 °C)	HQC	150.0	0.2	74.3	300.3	0.57	74.9
Dry extract (18 h at 10 °C)	LQC	50.0	0.61	97.0	100.15	1.92	100.9
Dry extract (18 h at 10 °C)	HQC	150.0	0.98	93.6	300.3	0.62	95.9