Potentials of N-(2, 4-dinitro-1-naphthyl)-p-toluenesulphonamide and 2, 4-dinitro-1-naphthol as novel charge transfer acceptors in pharmaceutical analysis of some quinolones and cephalosporins

Future Journal of Pharmaceutical Sciences

Table 3 Spot test results and chromatographic R_f values for 2, 4-DN1N-azo adduct formed with drugs studied

Drugs	Colour of adducts at 30 °C		Colour of adducts at 60 °C		R_f values** (CHCl₃: EtOAc: MeOH (4:4:2 v/v)
Drugs	Instantly	5 min	5 min	20 min	R_f values** (CHCl₃: EtOAc: MeOH (4:4:2 v/v)
Sparfloxacin	Golden yellow	Golden yellow	Golden yellow	Golden yellow	0.23 and 0.81 (0.23)
Norfloxacin	Golden yellow	Golden yellow	Golden yellow	Golden yellow	0.10 and 0.81 (0.10)
Ofloxacin	Golden yellow	Golden yellow	Golden yellow	Golden yellow	0.10 and 0.81 (0.10)
Ceftriaxone	Golden yellow	Golden yellow	Golden yellow	Golden yellow	0.13 and 0.81 (0.13)
Ceftazidime	Golden yellow	Golden yellow	Golden yellow	Golden yellow	0.10 and 0.81 (0.10)
Cefuroxime	Golden yellow	Golden yellow	Golden yellow	Golden yellow	0.10 and 0.81 (0.10)

*Blank reagent solution without drug is greenish yellow; **R_f of drug substance in parenthesis (R_f of reagent = 0.81)